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In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots <t>of</t> <t>Annexin</t> <t>V-FITC/PI</t> staining for <t>apoptosis</t> analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
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In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots <t>of</t> <t>Annexin</t> <t>V-FITC/PI</t> staining for <t>apoptosis</t> analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.
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SS-EVLP attenuates dexamethasone-induced loss of viability and <t>apoptosis</t> in MC3T3-E1 cells. ( a and d ) Effects of different concentrations of SS-EVLP on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( b and e ) Effects of different concentrations of dexamethasone (DEX) on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( c and f ) SS-EVLP-mediated restoration of cell viability in MC3T3-E1 cells exposed to 100 μM DEX at 24 and 48 h, respectively. ( g ) Representative flow cytometric plots <t>of</t> <t>Annexin</t> <t>V-FITC/PI</t> double staining in the indicated groups. ( h ) Quantification of apoptotic cells. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.
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In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Journal: International Journal of Pharmaceutics: X

Article Title: A pH-responsive dual-drug nanoplatform for stromal remodeling and enhanced chemotherapy via MMP3/TGF- β inhibition

doi: 10.1016/j.ijpx.2026.100489

Figure Lengend Snippet: In vitro therapeutic efficacy and synergy analysis of RPAE-QM in 4 T1 cells. (A) Cell viability of 4 T1 cells incubated with different formulations for 48 h determined by MTT assay. (B) The corresponding IC 50 values of the respective treatments. (C) Representative flow cytometry plots of Annexin V-FITC/PI staining for apoptosis analysis in 4 T1 cells. (D) Quantitative analysis of the total apoptotic rate. (E) Dose-response curves of free Que., free DM1, and their combination used for quantitative synergy determination. (F) The Combination Index (CI) plot as a function of Fraction affected (Fa) generated using the Chou-Talalay method; the reference line at CI = 1 indicates an additive effect, while CI < 1 indicates synergism. Data are presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001.

Article Snippet: After 24 h, the cells were harvested and stained with Annexin V-FITC/PI Cell Apoptosis Detection Kit (Beyotime, China) for flow cytometer analysis.

Techniques: In Vitro, Drug discovery, Incubation, MTT Assay, Flow Cytometry, Staining, Generated

SS-EVLP attenuates dexamethasone-induced loss of viability and apoptosis in MC3T3-E1 cells. ( a and d ) Effects of different concentrations of SS-EVLP on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( b and e ) Effects of different concentrations of dexamethasone (DEX) on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( c and f ) SS-EVLP-mediated restoration of cell viability in MC3T3-E1 cells exposed to 100 μM DEX at 24 and 48 h, respectively. ( g ) Representative flow cytometric plots of Annexin V-FITC/PI double staining in the indicated groups. ( h ) Quantification of apoptotic cells. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: International Journal of Nanomedicine

Article Title: Spatholobus suberectus Stem-Derived Extracellular Vesicle-Like Particles Attenuate Glucocorticoid-Induced Osteoporosis via NRF2/HO-1 Signaling

doi: 10.2147/IJN.S585928

Figure Lengend Snippet: SS-EVLP attenuates dexamethasone-induced loss of viability and apoptosis in MC3T3-E1 cells. ( a and d ) Effects of different concentrations of SS-EVLP on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( b and e ) Effects of different concentrations of dexamethasone (DEX) on MC3T3-E1 cell viability at 24 and 48 h, respectively. ( c and f ) SS-EVLP-mediated restoration of cell viability in MC3T3-E1 cells exposed to 100 μM DEX at 24 and 48 h, respectively. ( g ) Representative flow cytometric plots of Annexin V-FITC/PI double staining in the indicated groups. ( h ) Quantification of apoptotic cells. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA followed by Dunnett’s multiple-comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: Cell Counting Kit-8 (CCK-8) and Annexin V-FITC/PI apoptosis detection kits were purchased from Beyotime (Shanghai, China).

Techniques: Double Staining